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Chemical Constitution from Cell Walls of Bacteria: Fundamentals of Differential Staining
Developed by Castillo-Reyna, J. González-Barriga, C.D., Silveyra-Sáenz, G.A. and SEREX
Step 1: Fix a bacterial smear in the flame of a Bunsen burner.
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Step 2: Apply crystal violet on the slide and keep it for 60 seconds. Wash it with water.
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Step 3: Apply iodine on the slide and keep it for 60 seconds. Wash it with water.
Step 4: Apply 95% ethyl alcohol on the slide and keep it for 5 to 10 seconds. Wash it with water.
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Step 5: Finally, apply safranin on the slide and keep it for 45 seconds. Wash it with water
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Step 2: Apply crystal violet on the slide and keep it for 60 seconds. Wash it with water.
Step 2: Apply crystal violet on the slide and keep it for 60 seconds. Wash it with water.
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